Plasmid Preparation:Article Title: Pyrroloquinoline Quinone-Dependent Dehydrogenases from Ketogulonicigenium vulgare Catalyze the Direct Conversion of l -Sorbosone to l -Ascorbic Acid
Article Snippet: .. Strain DSM 4025, formerly classified in the genus Gluconobacter ( 35 ) and recently proposed to be reclassified in the new genus Ketogulonicigenium ( 34 ), was used for preparation of the SNDHs and chromosomal DNA. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain or plasmid Relevant characteristic(s) a Source or reference E. coli strains VCS257 Derivative of DP50 supF [ supE44 supF58 hsdS3 ( r B − m B − ) dapD8 lacY1 glnV44 Δ( gal-uvrB ) 47 tyrT58 gyrA29 tonA53 Δ( thyA57 )] Stratagene JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ( lac-proAB )/F′ [ traD36 proAB + lacI q lacZ Δ M15 ] Takara Bio DH5α F − φ80d lacZ Δ M15 Δ( lacZYA-argF ) U169 deoR recA1 endA1 hsdR17 (r K − m K + ) phoA supE44 λ − thi-1 gyrA96 relA1 Takara Bio HB101 leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ( mcrC-mrr ) Takara Bio M15 Nal s Str s Rif s Thi − Lac − Ara + Gal + Mtl − F − RecA + Uvr + Lon + QIAGEN DSM 4025 derivatives DSM 4025 K. vulgare strain 34 GOMTR1 Rif r , spontaneous mutant of DSM 4025 This study GOMTR1SN::Km Rif r Km r , sndH gene disruptant of GOMTR1 This study Plasmids pVK100 ATCC 37156, RK2 (IncP) derivative, 23 kb, Km r Tc r , cos ATCC b pRK2013 RK2 (IncP) derivative, 45 kb, Km r , mob ATCC pSUP202 Ap r Tc r Cm r , mob 28 pSUPSN pSUP202 carrying sndH This study pSUPSN::Km pSUP202 carrying sndH disrupted with Km r gene cassette This study pQE30 Six-His tag, Ap r QIAGEN pUC18/19 Ap r Takara Bio pUC4K Ap r Km r TOYOBO Open in a separate window a Ap r , ampicillin resistance; Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Tc r , tetracycline resistance; Rif r , rifampin resistance. b ATCC, American Type Culture Collection. .. Strain DSM 4025, formerly classified in the genus Gluconobacter ( 35 ) and recently proposed to be reclassified in the new genus Ketogulonicigenium ( 34 ), was used for preparation of the SNDHs and chromosomal DNA. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain or plasmid Relevant characteristic(s) a Source or reference E. coli strains VCS257 Derivative of DP50 supF [ supE44 supF58 hsdS3 ( r B − m B − ) dapD8 lacY1 glnV44 Δ( gal-uvrB ) 47 tyrT58 gyrA29 tonA53 Δ( thyA57 )] Stratagene JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ( lac-proAB )/F′ [ traD36 proAB + lacI q lacZ Δ M15 ] Takara Bio DH5α F − φ80d lacZ Δ M15 Δ( lacZYA-argF ) U169 deoR recA1 endA1 hsdR17 (r K − m K + ) phoA supE44 λ − thi-1 gyrA96 relA1 Takara Bio HB101 leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ( mcrC-mrr ) Takara Bio M15 Nal s Str s Rif s Thi − Lac − Ara + Gal + Mtl − F − RecA + Uvr + Lon + QIAGEN DSM 4025 derivatives DSM 4025 K. vulgare strain 34 GOMTR1 Rif r , spontaneous mutant of DSM 4025 This study GOMTR1SN::Km Rif r Km r , sndH gene disruptant of GOMTR1 This study Plasmids pVK100 ATCC 37156, RK2 (IncP) derivative, 23 kb, Km r Tc r , cos ATCC b pRK2013 RK2 (IncP) derivative, 45 kb, Km r , mob ATCC pSUP202 Ap r Tc r Cm r , mob 28 pSUPSN pSUP202 carrying sndH This study pSUPSN::Km pSUP202 carrying sndH disrupted with Km r gene cassette This study pQE30 Six-His tag, Ap r QIAGEN pUC18/19 Ap r Takara Bio pUC4K Ap r Km r TOYOBO Open in a separate window a Ap r , ampicillin resistance; Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Tc r , tetracycline resistance; Rif r , rifampin resistance. b ATCC, American Type Culture Collection.
Acetylene Reduction Assay:Article Title: Pyrroloquinoline Quinone-Dependent Dehydrogenases from Ketogulonicigenium vulgare Catalyze the Direct Conversion of l -Sorbosone to l -Ascorbic Acid
Article Snippet: .. Strain DSM 4025, formerly classified in the genus Gluconobacter ( 35 ) and recently proposed to be reclassified in the new genus Ketogulonicigenium ( 34 ), was used for preparation of the SNDHs and chromosomal DNA. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain or plasmid Relevant characteristic(s) a Source or reference E. coli strains VCS257 Derivative of DP50 supF [ supE44 supF58 hsdS3 ( r B − m B − ) dapD8 lacY1 glnV44 Δ( gal-uvrB ) 47 tyrT58 gyrA29 tonA53 Δ( thyA57 )] Stratagene JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ( lac-proAB )/F′ [ traD36 proAB + lacI q lacZ Δ M15 ] Takara Bio DH5α F − φ80d lacZ Δ M15 Δ( lacZYA-argF ) U169 deoR recA1 endA1 hsdR17 (r K − m K + ) phoA supE44 λ − thi-1 gyrA96 relA1 Takara Bio HB101 leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ( mcrC-mrr ) Takara Bio M15 Nal s Str s Rif s Thi − Lac − Ara + Gal + Mtl − F − RecA + Uvr + Lon + QIAGEN DSM 4025 derivatives DSM 4025 K. vulgare strain 34 GOMTR1 Rif r , spontaneous mutant of DSM 4025 This study GOMTR1SN::Km Rif r Km r , sndH gene disruptant of GOMTR1 This study Plasmids pVK100 ATCC 37156, RK2 (IncP) derivative, 23 kb, Km r Tc r , cos ATCC b pRK2013 RK2 (IncP) derivative, 45 kb, Km r , mob ATCC pSUP202 Ap r Tc r Cm r , mob 28 pSUPSN pSUP202 carrying sndH This study pSUPSN::Km pSUP202 carrying sndH disrupted with Km r gene cassette This study pQE30 Six-His tag, Ap r QIAGEN pUC18/19 Ap r Takara Bio pUC4K Ap r Km r TOYOBO Open in a separate window a Ap r , ampicillin resistance; Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Tc r , tetracycline resistance; Rif r , rifampin resistance. b ATCC, American Type Culture Collection. .. Strain DSM 4025, formerly classified in the genus Gluconobacter ( 35 ) and recently proposed to be reclassified in the new genus Ketogulonicigenium ( 34 ), was used for preparation of the SNDHs and chromosomal DNA. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain or plasmid Relevant characteristic(s) a Source or reference E. coli strains VCS257 Derivative of DP50 supF [ supE44 supF58 hsdS3 ( r B − m B − ) dapD8 lacY1 glnV44 Δ( gal-uvrB ) 47 tyrT58 gyrA29 tonA53 Δ( thyA57 )] Stratagene JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ( lac-proAB )/F′ [ traD36 proAB + lacI q lacZ Δ M15 ] Takara Bio DH5α F − φ80d lacZ Δ M15 Δ( lacZYA-argF ) U169 deoR recA1 endA1 hsdR17 (r K − m K + ) phoA supE44 λ − thi-1 gyrA96 relA1 Takara Bio HB101 leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ( mcrC-mrr ) Takara Bio M15 Nal s Str s Rif s Thi − Lac − Ara + Gal + Mtl − F − RecA + Uvr + Lon + QIAGEN DSM 4025 derivatives DSM 4025 K. vulgare strain 34 GOMTR1 Rif r , spontaneous mutant of DSM 4025 This study GOMTR1SN::Km Rif r Km r , sndH gene disruptant of GOMTR1 This study Plasmids pVK100 ATCC 37156, RK2 (IncP) derivative, 23 kb, Km r Tc r , cos ATCC b pRK2013 RK2 (IncP) derivative, 45 kb, Km r , mob ATCC pSUP202 Ap r Tc r Cm r , mob 28 pSUPSN pSUP202 carrying sndH This study pSUPSN::Km pSUP202 carrying sndH disrupted with Km r gene cassette This study pQE30 Six-His tag, Ap r QIAGEN pUC18/19 Ap r Takara Bio pUC4K Ap r Km r TOYOBO Open in a separate window a Ap r , ampicillin resistance; Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Tc r , tetracycline resistance; Rif r , rifampin resistance. b ATCC, American Type Culture Collection.
Mutagenesis:Article Title: Pyrroloquinoline Quinone-Dependent Dehydrogenases from Ketogulonicigenium vulgare Catalyze the Direct Conversion of l -Sorbosone to l -Ascorbic Acid
Article Snippet: .. Strain DSM 4025, formerly classified in the genus Gluconobacter ( 35 ) and recently proposed to be reclassified in the new genus Ketogulonicigenium ( 34 ), was used for preparation of the SNDHs and chromosomal DNA. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain or plasmid Relevant characteristic(s) a Source or reference E. coli strains VCS257 Derivative of DP50 supF [ supE44 supF58 hsdS3 ( r B − m B − ) dapD8 lacY1 glnV44 Δ( gal-uvrB ) 47 tyrT58 gyrA29 tonA53 Δ( thyA57 )] Stratagene JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ( lac-proAB )/F′ [ traD36 proAB + lacI q lacZ Δ M15 ] Takara Bio DH5α F − φ80d lacZ Δ M15 Δ( lacZYA-argF ) U169 deoR recA1 endA1 hsdR17 (r K − m K + ) phoA supE44 λ − thi-1 gyrA96 relA1 Takara Bio HB101 leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ( mcrC-mrr ) Takara Bio M15 Nal s Str s Rif s Thi − Lac − Ara + Gal + Mtl − F − RecA + Uvr + Lon + QIAGEN DSM 4025 derivatives DSM 4025 K. vulgare strain 34 GOMTR1 Rif r , spontaneous mutant of DSM 4025 This study GOMTR1SN::Km Rif r Km r , sndH gene disruptant of GOMTR1 This study Plasmids pVK100 ATCC 37156, RK2 (IncP) derivative, 23 kb, Km r Tc r , cos ATCC b pRK2013 RK2 (IncP) derivative, 45 kb, Km r , mob ATCC pSUP202 Ap r Tc r Cm r , mob 28 pSUPSN pSUP202 carrying sndH This study pSUPSN::Km pSUP202 carrying sndH disrupted with Km r gene cassette This study pQE30 Six-His tag, Ap r QIAGEN pUC18/19 Ap r Takara Bio pUC4K Ap r Km r TOYOBO Open in a separate window a Ap r , ampicillin resistance; Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Tc r , tetracycline resistance; Rif r , rifampin resistance. b ATCC, American Type Culture Collection. .. Strain DSM 4025, formerly classified in the genus Gluconobacter ( 35 ) and recently proposed to be reclassified in the new genus Ketogulonicigenium ( 34 ), was used for preparation of the SNDHs and chromosomal DNA. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain or plasmid Relevant characteristic(s) a Source or reference E. coli strains VCS257 Derivative of DP50 supF [ supE44 supF58 hsdS3 ( r B − m B − ) dapD8 lacY1 glnV44 Δ( gal-uvrB ) 47 tyrT58 gyrA29 tonA53 Δ( thyA57 )] Stratagene JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ( lac-proAB )/F′ [ traD36 proAB + lacI q lacZ Δ M15 ] Takara Bio DH5α F − φ80d lacZ Δ M15 Δ( lacZYA-argF ) U169 deoR recA1 endA1 hsdR17 (r K − m K + ) phoA supE44 λ − thi-1 gyrA96 relA1 Takara Bio HB101 leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 Δ( mcrC-mrr ) Takara Bio M15 Nal s Str s Rif s Thi − Lac − Ara + Gal + Mtl − F − RecA + Uvr + Lon + QIAGEN DSM 4025 derivatives DSM 4025 K. vulgare strain 34 GOMTR1 Rif r , spontaneous mutant of DSM 4025 This study GOMTR1SN::Km Rif r Km r , sndH gene disruptant of GOMTR1 This study Plasmids pVK100 ATCC 37156, RK2 (IncP) derivative, 23 kb, Km r Tc r , cos ATCC b pRK2013 RK2 (IncP) derivative, 45 kb, Km r , mob ATCC pSUP202 Ap r Tc r Cm r , mob 28 pSUPSN pSUP202 carrying sndH This study pSUPSN::Km pSUP202 carrying sndH disrupted with Km r gene cassette This study pQE30 Six-His tag, Ap r QIAGEN pUC18/19 Ap r Takara Bio pUC4K Ap r Km r TOYOBO Open in a separate window a Ap r , ampicillin resistance; Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Tc r , tetracycline resistance; Rif r , rifampin resistance. b ATCC, American Type Culture Collection.
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